Abstract
A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico.
Highlights
Lactic acid bacteria (LAB) have been widely studied for their various applications as probiotics and food products
Studies have shown that lactic acid bacteria have a vast amount of bioactive compounds that are beneficial as antifungal [3], antibiotic-resistant uropathogens [3], and other human pathogenic bacteria [4], in addition to other potential uses such as anti-cancer, anti-hypertensive, anti-thrombotic, lowering of cholesterol [5], anti-oxidant, and immunomodulation from food proteins [6] in the receptors of the gut epithelium [7]
Twenty active LAB isolates were selected based on the profile of hydrogen peroxide production and tested against A. baumannii ATCC 19606, A. haemolyticus ATCC 19002, A. iwoffii ATCC 15309, and XDR A. baumannii
Summary
Lactic acid bacteria (LAB) have been widely studied for their various applications as probiotics and food products. Culture supernatants from LAB were previously characterized for inhibition of bacteria and fungi and further attributed to the production of antifungal peptides in apple [1]. This study will need to characterize the lactic acid bacteria to survive GI tract conditions, production of antimicrobial substances, tolerance to gastric acid and bile, and ability to adhere or co-aggregate to the intestinal epithelial cells as well as evaluated for the possible transferable antibiotic resistance prior to human clinical trials [8]
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