Abstract

Olfactory perception is mediated by a large array of olfactory receptor genes. The human genome contains 851 olfactory receptor gene loci. More than 50% of the loci are annotated as nonfunctional due to frame-disrupting mutations. Furthermore haplotypic missense alleles can be nonfunctional resulting from substitution of key amino acids governing protein folding or interactions with signal transduction components. Beyond their role in odor recognition, functional olfactory receptors are also required for a proper targeting of olfactory neuron axons to their corresponding glomeruli in the olfactory bulb. Therefore, we anticipate that profiling of olfactory receptor gene expression in whole human olfactory mucosa and analysis in the human population of their expression should provide an opportunity to select the frequently expressed and potentially functional olfactory receptors in view of a systematic deorphanization. To address this issue, we designed a TaqMan Low Density Array (Applied Biosystems), containing probes for 356 predicted human olfactory receptor loci to investigate their expression in whole human olfactory mucosa tissues from 26 individuals (13 women, 13 men; aged from 39 to 81 years, with an average of 67±11 years for women and 63±12 years for men). Total RNA isolation, DNase treatment, RNA integrity evaluation and reverse transcription were performed for these 26 samples. Then 384 targeted genes (including endogenous control genes and reference genes specifically expressed in olfactory epithelium for normalization purpose) were analyzed using the same real-time reverse transcription PCR platform. On average, the expression of 273 human olfactory receptor genes was observed in the 26 selected whole human olfactory mucosa analyzed, of which 90 were expressed in all 26 individuals. Most of the olfactory receptors deorphanized to date on the basis of sensitivity to known odorant molecules, which are described in the literature, were found in the expressed olfactory receptors gene set.

Highlights

  • Analysis of published mammalian genomes indicates that olfactory receptor (OR) genes constitute by far the largest gene family

  • The OR gene family was discovered over 20 years ago by Buck and Axel, few data are available on their expression in human olfactory mucosa, contrasting with the recent significant increase of results on the genetic polymorphism of OR genes [48]

  • We report here the first extensive high throughput transcriptome profiling of OR gene expression directly by real-time reverse transcription PCR performed on whole human olfactory mucosa (WHOM) from a relatively large population of 26 patients

Read more

Summary

Introduction

Analysis of published mammalian genomes indicates that olfactory receptor (OR) genes constitute by far the largest gene family. Buck and Axel identified this extremely large multigene family based on the observation that OR genes were expressed in olfactory epithelium of rat [1]. More than 50% of the loci are annotated as nonfunctional due to frame-disrupting mutations, leaving approximately 400 potentially functional OR genes. In spite of this rather accurate genomic characterization, very little is known of the functional and integrative mechanisms of human olfactory receptor in odorant perception. The responses of only 48 human ORs with one or more odorant molecules have been reported [5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20] and less than ten of these receptors have been reliably associated with olfactory perception of an odorant stimuli [8,9,10,13,14]

Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call