Profiling of genes in healthy hGF, aging hGF, healthy hPDLF and inflammatory hPDLF by DNA microarray

Abstract

One of the main goals of periodontal treatment is to regenerate tissue from the periodontal ligament (PDL). Ideal periodontal healing is achieved by the promotion of PDL cells 1-4) . PDL cells have a potential to promote tissue regeneration through their various activities, such as migration, proliferation, osteoblast-like differentiation, cementoblast-like differentiation, and periodontal ligament fibroblasts. The main purpose in periodontal regeneration therapy is to regenerate periodontal tissue with human periodontal ligament fibroblast (hPDLF) being essential in this process.1) Melcher et al 5) . reported that the phenotypes of cells re-collected from the root surface (such as gingival epithelium, gingival lamina propria, periodontal ligament, cementum, and alveoloar bone) determine the characteristics and quality of regeneration. This theory forms the biological basis of guided tissue regeneration (GTR). For periodontal regeneration, the ability of hPDLF cells to divide into various cells is important. Comparing the expression of hPDLF in the presence of a periodontal infection is essential for determining if the functions and roles of hPDLF cells can be used in periodontal regeneration therapy. The most abundant cell in periodontal connective tissues is the gingival fibroblast. Periodontal ligament fibroblasts (PDLF) and gingival fibroblasts (GF) have distinct functional activities in the regeneration and repair of periodontal tissues as well as in inflammatory periodontal diseases 6-10) . Generally, the teeth extracted from severe periodontitis patients have no PDL. It should be noted that hGF can be