Profiling analysis of differentially expressed genes in colorectal cancer with or without high expression of gastrin

Abstract

Objective To screen the differentially expressed genes between colorectal cancers with high-and no-gastrin expression by using cDNA array analysis in order to find new candidate genes related to gastrin-based colorectal cancers.Methods Colorectal cancers were collected from 43 patients,and gastrin was determined by using enzyme linked immunosorbent assay (ELISA).Human colorectal cancers with high-and no-gastrin expression were analyzed by using the Whole Genome Oligo Arrays.Array data of 10 differential genes which may play important roles in signaling pathways were confirmed using real-time polymerase chain reaction (PCR).Results There were significant differences in gene expression between human colorectal cancer with high-and no-expression gastrin.Among the target genes,1182 differentially expressed genes were identified.As compared to colorectal cancers without gastrin expression,738 differentially expressed genes were up-regulated and the remaining 444 were down-regulated in colorectal cancers,including cell growth and proliferation-related genes,cancer metastasis-related genes,as well as genes related to cytoskeleton structure which focused on the Wnt and other signaling pathways.We selected 10 differential genes which may play important roles in the signaling pathways and verified these genes by realtime PCR,and the results were highly consistent with cDNA array data.Conclusion There were different gene expression profiles between human colorectal cancers with high-and no-gastrin expression.These genes may provide important clues for finding new genes related to gastrin-based colorectal cancers. Key words: Colorectal carcinoma; Gastrin ; Gene