Profiling analysis of differential gene expression between breast cancer cell sublines with high- and low-metastatic potential

Abstract

Objective To establish variant cell sublines expressing green fluorescent protein with high- and low-metastatic potential, and investigate gene expression profile in these cell sublines in order to find new candidate genes related to metastasis of breast cancer to bone. Methods Twenty μg total RNA of MCF7 cell sublines A2 and C6 was extracted, purified to mRNA and then reversely transcripted to cDNA probe which labeled with Cy5 and Cy3 fluorescence respectively. The two samples were hybridized with the cDNA microarray chips containing 7458 human target genes. The hybridization signals were scanned by Generation Ⅲ array scanner and obtained data were analyzed using Imagequant 5. 0 and Array Vision 6. 0 software. Results There were significant differences in gene expression between A2 and C6 cell sublines.Among the target genes, 767 differentially expressed genes were identified. As compared to A2, 480 of 767 differentially expressed genes were up-regulated and the remaining 287 were down-regulated. Conclusion There were different gene expression profiles between high- and low-metastatic potential cell sublines.These genes may provide important clues for finding genes related to metastasis of breast cancer to bone. Key words: Breast carcinoma; Metastasis; cDN A microarray; Gene