Profiles of Vibrio and heterotrophic bacteria in the intensive Vanamae shrimp culture using bioremediation technique in Karawang

Abstract

Vibrio sp. group is one of the parameters of the health level of a shrimp culture. The aim of this study is to learn the population of vibrio and heterotrophic bacteria in Vaname shrimps’ cultivation under the use of probiotic bacteria. The profile analysis of the Vibrio sp. and heterotrophic bacteria were done in the Vanname shrimp culture with implementation of selected bacteria as bioremediation agents in Balai Layanan Usaha Budidaya Perikanan, Karawang. The studies were conducted in 110 days using the culture density of 60 shrimps/m2. Three ponds of 2,500 m2 namely A5, A7 and B5 ponds were set up for the observation and sample-taking for nine times. The Vibrio sp. and heterotrophic bacteria were analyzed using a plate-count technique with Thiosulphate Citrate Bile-salt Sucrose (TCBS) agar and Sea-Water Complete (SWC) media respectively. The shrimp’s growth and survival rate, and the water quality parameters were also monitored periodically. The result showed that Vibrio sp. population were fluctuated. The population was between 1.4-29.0 x 102 CFU/mL. The result showed that Vibrio sp. population were fluctuated. The highest Vibrio population was 103 CFU/mL while the highest profile of heterotrophic bacteria around 105 CFU/mL in average. The shrimp weight in final day of culture showed positive increase with the average body weight of 22.34±0.68 g/indv, while the average daily growth rate was 0.31±0.01 % g/day and the average of survival rate in the final experiment was 58.42±1.50%. The water quality was fluctuated by increasing days of culture. The temperature of water during culture periode ranged between 26.46 ± 1.87°C to 26.97 ± 0.87°C, with the pH value ranged from 7.31 ± 0.36 to 7.59 ± 0.28 and the salinity ranged between 24.23 ± 43.87 to 24.61 ± 4.15 ppt.