Abstract
The development of endoscopic retrograde cannulation of the pancreatic duct allows collection of pure pancreatic juice uncontaminated by bile and duodenal secretions. We have analyzed such pure pancreatic juice to establish secretory profiles of some normal components. Fifteen healthy human volunteers were studied. Secretin (1 clinical unit per kg) was injected intravenously at time 0, and 16 min later cholecystokinin-pancreozymin (CCK-PZ) (1 Ivy dog unit per kg) was injected intravenously. Pure pancreatic juice was collected for 32 min in timed minute samples. Secretin produced an almost immediate and sustained flow of juice (3.15 ± 0.24 ml per min) with initial high concentrations of protein and enzyme. After CCK-PZ, flow increased significantly to 3.40 ± 0.29 ml per min (P < 0.02) and enzyme concentration rose some 10-fold above that found during secretin stimulation. The maximum of the CCK-PZ effect occurred 2 to 3 min after injection and its half-life was 73 to 90 sec for total protein and enzymes but only 64 sec for trypsin inhibitor. Repeat studies in 3 subjects indicate marked reproducibility for these various parameters. Trypsinogen and proelastase appeared to be secreted in a parallel fashion but nonparallel secretion for amylase, chymotrypsin, and trypsin inhibitor was observed. Amylase secretion was proportionately less and trypsin inhibitor secretion proportionately more stimulated by CCK-PZ than was chymotrypsinogen. Collections of single minute samples of pure pancreatic juice reveal a more accurate profile of secretory events than does collection of pooled samples for 10 to 20 min as hitherto performed by other workers.
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