Profiles of Human Papillomavirus Detection of the Multinucleated Cells in Cervical Smears.

Kaori Okayama,Koji Teruya,Toshiyuki Sasagawa,Hirokazu Kimura,Mizue Oda,Mitsuaki Okodo,Masahiko Fujii

doi:10.3390/microorganisms9081575

Abstract

Many genotypes of human papillomaviruses (HPVs) may lead to morphological changes in cells, resulting in various atypical cells, such as multinucleated cells (MNCs) and koilocytes, in the cervix. However, the relationships between the profiles of HPV genotypes and MNCs are not exactly known. Thus, this study comprehensively profiles the HPV genotypes in MNCs using a microdissection method. HPV genotypes and MNCs were detected in 651 cases with an abnormal Pap smear by liquid-based cytology. Specific HPV genotypes were also detected, including HPV16, 34, and 56, which might be associated with MNCs. This result suggests that the high-risk HPV genotypes, such as HPV16 and 56, are associated with the atypical changes in MNC morphology from normal cervical cells. The results also show that MNCs may be a predictor of squamous intraepithelial lesion.

Highlights

The relationships among the multinucleated cells (MNCs), cytologic classifications, and human papillomaviruses (HPVs) genotypes are summarized in Tables 1–3, respectively
We studied the relationships between MNC, cytopathological classification by Bethesda system, and HPV infection profiles in cervical smears stained using the Pap and microdissection methods
Various low-risk and high-risk HPV genotypes were detected in the whole cells, while common genotypes, such as HPV genotype 16 (HPV16) and 56, were detected in MNCs (Table 4)

Summary

Introduction

Some of them are cancer-associated genotypes (high-risk types), including HPV genotype 16 (HPV16), HPV18, and HPV52 [3,4]. Previous studies have suggested that these high-risk genotypes are the leading causes of cervical cancers [5,6]. Various atypical cells, including koilocytes and multinucleated cells (MNCs), have been confirmed in cervical smears [7]. The change from a normal cell to an atypical cell may be due to HPV infection [7]. Our previous study suggested that many HPV genotypes were detected in koilocytes, and their morphological changes may be due to these HPV infections [8]. MNC is reportedly a predictor of HPV-infected cells [9]. The relationships between the profiles of the HPV genotypes and MNCs are not well-understood

Methods

Results

Conclusion