Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

E I Ermolenko,A B Karaseva,Yu D Kondratenko,I V Kudryavtsev,M K Serebryakova,M P Kotyleva,A N Tsapieva,A N Suvorov,I A Shumikhina,O I Solovyova

doi:10.15789/1563-0625-pos-1905

E I Ermolenko, A B Karaseva + Show 8 more

Open Access

https://doi.org/10.15789/1563-0625-pos-1905

Copy DOI

Journal: Medical Immunology (Russia)	Publication Date: Apr 16, 2020
Citations: 2	License type: cc-by

Affiliation: St Petersburg University

Abstract

The following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including representatives of Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (Enterobacteriaceae and Desulfovibrionaceae families); 2) a decrease in the population of Bacteroidetes, including representatives of the families Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.). Firmicutes belonging to the families Clostridiaceae and Ruminococcaceae (Fecalibacterium spp.). Flow cytometry in the study of the subpopulation composition of T regulatory (Treg) lymphocytes in patients with IBS revealed an increase in the number of CD45R0+CD62L+ central memory cells (CM), which can regulate the processes of maturation and differentiation of lymphocytes in lymphoid tissue. A decrease in the expression of exonucleases CD39 and CD73 was detected, which can have a significant effect on their activity. A reduction in effector memory cells (EM) Treg was observed. Changes in the expression level of exonucleases CD39 and CD73 were inversely correlated with the content of Proteobacteria and the representation of the genera Bifidobacterium spp. and Faecalibacterium spp. The content of СЫ Treg was directly correlated with the content of Dorea spp. The results may be indicative of impairment in the processes of Treg differentiation, which are closely related to changes in key components of intestinal microbiocenosis in IBS.

Highlights

Irritable bowel syndrome (IBS) is one of the most common functional diseases of the gastrointestinal tract, which affects more than 40% of gastroenterology patients
IBS increased the population of enterobacteria [36], lipopolysaccharides (LPS) of the cell wall, which can have a direct effect on immunocompetent cells, interacting with TLR4 receptors on their surface [20, 29]
Metagenomic analysis showed that at the filum level there is an increase in the representation of Actinobacteria, Firmacutes and Proteobacteria

Summary

Introduction

Irritable bowel syndrome (IBS) is one of the most common functional diseases of the gastrointestinal tract, which affects more than 40% of gastroenterology patients. The cellular composition and morphofunctional characteristics of the intestinal mucosa in IBS have been thoroughly studied. This disease, a condition in the gastrointestinal tract, is more often interpreted not as dysbiosis, but as “low-grade inflammation” [4, 37]. The microbiota of IBS patients demonstrated an increase in the representation of phylum Proteobacteria, changes in the ratio of individual representatives of phylum Bacteroidetes and Firmicutes, a decrease in biodiversity [25, 26, 36]. IBS increased the population of enterobacteria [36], lipopolysaccharides (LPS) of the cell wall, which can have a direct effect on immunocompetent cells, interacting with TLR4 receptors on their surface [20, 29]

Objectives

Results

Conclusion