Abstract
Electronic cigarette is one type of cigarette that are popular. This is because of the nicotine content in electronic cigarette liquid that can be regulated by the user so it is considered safe. The purpose of this study was to analize electronic cigarette effect on hematology profile of male Balb’C mice (Mus musculus L). This study used 24 males mice which were divided into 4 groups. Group 1 (control) was the group without electronic cigarette exposure. The group 2,3, and 4 were exposed to electronic cigarette for 1 ml, 2 ml and 4 ml liquid respectively. This study use liquid that containing 6 mg nicotin/ml. The exposure to electronic cigarette were conducted for 4 weeks. Hematology profile observation include hemoglobin level, erytrocytes count, count and types of leukocytes.The result showed that hemoglobin level and erytrocytes count was increase, leukocytes and lymphocyte count was decrease and no effect on granulocytes and MID after electronic cigarette exposure using different liquid volume. The conclusion of this study was electronic cigarette exposure with different liquid volume as long as four weeks affect to Balb’C male mice hematology profile.
Highlights
E-cigarettes are a type of cigarette that is becoming increasingly popular. This is because the nicotine content in e-cigarette liquid can be regulated by the user so that it is considered safe
Hematology profile observations were carried out using a hematology analyzer
The results showed that after exposure to e-cigarette smoke with different fluid volumes there was an increase in hemoglobin and erythrocyte counts, a decrease in the number of leukocytes and lymphocytes and no change in the number of granulocytes and MID
Summary
Penelitian dilaksanakan pada bulan September sampai dengan Desember 2019 di Laboratorium Zoologi Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember dan Laboratorium Prosenda Jember. Alat yang digunakan untuk pemaparan asap rokok elektrik pada mencit dapat dilihat pada Gambar 1. Alat-alat yang digunakan untuk proses pemaparan asap rokok elektrik pada mencit (a) vaping box; (b) vapor; (c) aerator. Kelompok dua dipapar asap rokok elektrik dengan volume cairan sebanyak 1 ml. Kelompok tiga dipapar asap rokok elektrik dengan volume cairan sebanyak 2 ml. Kelompok empat dipapar asap rokok elektrik dengan volume cairan sebanyak 4 ml. Paparan asap rokok elektrik dilakukan selama 28 hari di dalam vaping box yang diberi lubang udara (aerator) dan lubang untuk paparan asap rokok elektrik (vapor) seperti tampak pada Gambar 1. Darah yang diambil melalui orbital mata sebanyak 100-300 μl dimasukkan dalam tabung yang mengandung K3EDTA.selanjutnya dilakukan pemeriksaan hematologi menggunakan hematologi analyzer sedangkan darah yang diambil melalui ekor dibuat preparat apus darah untuk pengamatan jenis-jenis leukosit
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