Abstract

This research is conducted to find out the genetic profiles of Kayuan clan which is belong to Pasek Catur Sanak Bali Mula, indigenous Balinese people. The DNA profiles is identified by using Microsatellite DNA of Y chromosome. Genes or DNA in Y chromosome are descended from father to son (patrilinealism). Four loci of microsatellite DNA from Y chromosome are used, including DYS19, DYS390, DYS393 and DYS395 to amplified DNA samples from 67 males probands. The probands are people of Kayuan clan from Siakin, Songan, Blandingan and Kutuh villages, Kintamani sub district and others are originally from Jehem village, Tembuku sub district, Bangli regency. The research found 12 allele varieties from all the analyzed loci, which resulted in low genetic diversity (0.33 + 0.001). There are 4 combinations alleles of 4 loci that created12 various haplotypes, the greatest one is haplotype 1 (0.39), followed by haplotype 2 (0.16), haplotype 7 (0,12) and the smallest one is haplotype 6 (0,10). On the other hand, eight other alleles found on the smaller frequencies. Two similar allele combinations were also found on DNA profiles of Celagi clan, which is also belong to the similar family, Pasek Catur Sanak Bali Mula. The two allele are haplotype 6 and 5 found in both, Pasek Kayuan and Pasek Celagi. The haplotype 6 as dominant allele of Celagi clan by adoption, haplotype 5 however, the frequency was the lowest. This allele only detected in one member of each clan due to mutation.

Highlights

  • This research is conducted to find out the genetic profiles of Kayuan clan

  • which is belong to Pasek Catur Sanak Bali Mula

  • Hlm. 57–66 identified by using Microsatellite DNA of Y chromosome

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Summary

Sanak Bali Mula

I Ketut Junitha, Ni Luh Watiniasih, dan Ni Luh Putu Ria Puspitha Universitas Udayana Email: Abstrak Tulisan ini mengkaji tentang profil genetika masyarakat soroh Kayuan yang termasuk dalam klan Pasek Catur Sanak Bali Mula menggunakan marka DNA mikrosatelit pada kromosom Y. Hasil penelitian menunjukkan bahwa ditemukan seba­nyak 12 ragam alel dari semua lokus yang digunakan de­ngan keragaman genetik yang rendah hanya sebesar 0,33 ± 0,001. Berdasarkan kombinasi alel-alel semua lokus ditemukan profil genetik laki-laki sebanyak 12 haplotipe yang didominasi oleh haplotipe 1 sebesar 0,39 diikuti oleh haplotipe 2 sebesar 0,16, haplotipe 7 sebesar 0,12 dan haplotipe 6 sebesar 0,10, sedangkan delapan haplotipe lainnya dengan frekuensi kecil-kecil. Ditemukan profil DNA pada laki-laki soroh Kayuan sama dengan profil DNA pada soroh Celagi sesama klan Pasek Catur Sanak Bali Mula yaitu haplotipe 6 yang merupakan haplotipe dominan pada soroh Celagi karena adopsi, sedangkan untuk haplotipe 5 sama-sama dengan frekuensi rendah yang mungkin akibat mutasi.

Ragam alel
Keragaman genetik paling tinggi ditemukan pada lokus
Daftar pustaka
Full Text
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