Abstract
Seeds of Cattleya walkeriana and Cyrtopodium palmjfrons ( Orchidaceae ) were aseptically transferred to culture medium Knudson, MS (Murashige& Skoog) and MS supplemented with IAA (l, 15 and 30 mg L -1 ) and kinetine (0.25, 5 and 10 mg L -1 ) for germination. They were kept at room temperature (23 +/- 2°C) under fluorescent light for 30 to 45 days. The seedlings were transferred to pots containing culture medium Knudson and incubated for four months at room temperature and transferred to fresh culture medium until the seedlings attained approximately 1.0 to 2.0 cm size for climatization. The in vitro cultivation of both plant species was considered viable. However the performance of the two orchids differed in relation to protocorms oxidation levels and in vitro growth. The results showed that less concentrated culture medium such as Knudson can be utilized , without the addition of growth regulators for the production of Cattleya walkeriana as well as Cyrtopodium palmifrons . KEY-WORDS: Orchidaceae ; tissue culture; orchid.
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