Abstract
Passiflora cincinnata is a wild species of passion fruit with a wide geographical distribution. It has vigorous growth, climbing habit and very showy and fragrant flowers. The aim of the present investigation was to obtain synthetic seeds from encapsulated zygotic and somatic embryos of P. cincinnata, cultivated under different conditions. Precotyledonary and cotyledonary stage embryos were obtained from zygotic embryos cultivated on MS medium supplemented with 18.1 μM of 2,4-Acid-dichlorophenoxyacetic (2,4-D) and 4.5 μM of Benzyladenine (BA). Zygotic embryos and somatic embryos stages were encapsulated using sodium alginate (2.5% w v-1) and CaCl2.2H2O (1 mM) as complexing agent. The zygotic and somatic embryos were encapsulated in a matrix containing (I) sodium alginate, (II) sodium alginate + artificial endosperm and (III) sodium alginate + artificial endosperm supplemented with activated charcoal (0.15% w/v). Zygotic embryos encapsulated in the matrix (I), matrix (II) and matrix (III) and cultivated in flasks, germinated at rates of 79%, 76% and 86% respectively. The cotyledonary somatic embryos encapsulated in the 3 different matrices showed better germination rates when cultivated on cellulose plugs, with more than 50% of embryos converted into plants. Precotyledonary somatic embryos did not germinated regardless the matrix and cultivation. When cultivating the alginate beads ex vitro, both substrate Plantmax and Florialite showed low number of germinated embryos, and the best result (12.67%) were obtained using Florialite and embryos encapsulated in the matrix (I).
Highlights
Synthetic seeds of a wild passionfruit species with ornamental potential Passiflora cincinnata is a wild species of passion fruit with a wide geographical distribution
No cultivo de sementes sintéticas de P. cincinnata sob as condições in vitro e ex vitro, em todos os tratamentos foram obtidos resultados semelhantes quanto aos embriões somáticos encapsulados somente na matriz de alginato de sódio, apresentando maior índice de germinação e conversão de plantas com desenvolvimento normal
Germination of synthetic seeds from the somatic embryos at cotyledonary stage of a P. cincinnata cultivaded in substrates Plantmax® e FlorialiteTM ex vitro. (I) - Encapsulated of zygotic embryos in the presence sodium alginate of a 2.5% (w v-1). (II) - Encapsulated of somatic embryos with artificial endosperm. (II) - Encapsulated of somatic embryos with artificial endosperm and supplemented with activated charcoal with 0.15% (w v-1)
Summary
Produção de sementes sintéticas de maracujazeiro silvestre com potencial ornamental por planta no cultivo in vitro (WEST et al, 2006; SILVA et al, 2014). O encapsulamento de propágulos com alginato de sódio é uma técnica que pode ser utilizada, associada à propagação in vitro para a conservação de inúmeras espécies de plantas (WEST et al, 2006). Passiflora cincinnata Masters é uma importante espécie silvestre de maracujazeiro, sendo utilizada como portaenxerto compatível para P. edulis por apresentar resistência à doença da parte aérea causada pela bactéria Xanthomonas axonopodis f. Esta espécie de maracujazeiro apresenta excelentes respostas morfogênicas com elevado potencial organogênico e embriogênico (OTONI et al, 2103), e tem sido considerada uma espécie modelo para estudos da morfogênese in vitro do gênero Passiflora (ROCHA et al, 2012). O trabalho teve como objetivo a produção de sementes sintéticas de Passiflora cincinnata utilizando como fonte de propágulos embriões somáticos e zigóticos em diferentes formas de cultivo
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
