Abstract
Lipase production by bacterial isolates from petroleum contaminated soil. This paper aims to present alternatives to the production of lipolytic enzymes from microorganisms isolated from soil with a history of contact with oil from the region of Sergipe, by submerged fermentation using palm oil as inducer. The experiments were conduced in 250 mL Erlenmeyer flasks in a shaker (170 rpm), containing 125 mL (%, w v -1 ) of KH 2 PO 4 , MgSO 4 .7H 2 O, NaNO 3 , yeast extract, peptone and starch. The pH was 5.0 and 7.0; incubation temperature was 30 and 37°C, respectively; fermentation time was 24, 48, 72, 96h, 120 and 144h; and the inducer concentration was 3 and 4%, added after 72h of fermentation. The determination of lipolytic activity was examined at 37°C, in a shaker in thermostatic bath with rotation of 82 rpm, using gum arabic 7% (w v -1 ), olive oil and water. The reactions were conducted for 10 min with agitation and later stopped by the addition of an acetone:ethanol:water solution, where released fatty acids were titrated with a solution of KOH (0.04 N), using phenolphthalein as an indicator. The maximum activity was determined as 4369.75 U mL -1 pH 7.0 and a temperature of 37°C, with the percentage of the inducer oil palm to 4%.
Highlights
NaNO3, extrato de levedura, peptona e amido
The maximum activity was determined as 4369.75 U mL-1 pH 7.0 and a temperature of 37°C, with the percentage of the inducer oil palm to 4%
Foram utilizados microrganismos isolados de solos contaminados por petróleo (Biopetro IV) e pertencentes ao Laboratório de Engenharia de Bioprocessos do Instituto de Tecnologia e Pesquisa da Universidade Tiradentes, Farolândia, Estado de Sergipe
Summary
A fermentação foi conduzida em frascos erlenmeyer de 250 mL, em agitador do tipo shaker, com velocidade de 170 rpm; a 30 e 37oC, durante 144h, com 125 mL (%, p v-1) de: KH2PO4, MgSO4.7H2O, NaNO3, extrato de levedura, peptona e amido. As condições da fermentação foram: pH 5,0 e 7,0; temperatura de 30 e. 37oC de incubação; e velocidade de agitação (170 rpm). As análises do substrato fermentado foram conduzidas, retirando-se 8 mL de amostra, centrifugada a 3.000 rpm por 15 min., sendo o sobrenadante utilizado para determinação da atividade enzimática. A suspensão foi usada para a determinação de pH, da dosagem de amido, da dosagem de proteína e da dosagem da atividade lipolítica. O peso seco foi determinado a partir das mesmas suspensões, realizando-se centrifugação e secagem das amostras em estufa a 110°C, até peso constante (LIMA; ANGNES, 1997)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
