Products with biological activity obtained from in vitro micropropagated Sideritis foetens

Abstract

A method for the in vitro micropropagation of Sideritis foetens species is described. About one million plants were produced from a single nodal segment in one year. Phytochemical studies of plants indicated that this method increases their diterpene content, although the same type of products as in natural plants are obtained. ent-6α- Acetoxy-3β,8α-dihydroxylabda-13(16),14-diene, ent-6α,8α,18-trihydroxylabda-13(16),14-diene, ent-6α-acetoxy-8α-18- dihydroxy-labda-13(16),14-diene and ent-18-acetoxy-6α,8α-dihydroxylabda-13(16),14-diene were isolated from micropropagated plants. ent-3β,6β-Dihydroxy-13- epi-manoyl oxide and ent-16,18-dihydroxy-6-oxomanoyl oxide (ketomanoyl) were obtained by chemical methods from these natural products. Both products inhibit basal adenylatecyclase activity and activity previously stimulated by forskolin or fluoride, but were without effect on glucagon-enhanced activity.