Productive infection of Autographa californica nucleopolyhedrovirus in silkworm Bombyx mori strain Haoyue due to the absence of a host antiviral factor

Abstract

We have reported that several silkworm strains are permissive to intrahemocoelical infection of Autographa californica nucleopolyhedrovirus (AcNPV), contrary to the general belief that AcNPV cannot infect silkworm. In the present study, we address whether the intrahemocoelical infection of AcNPV to the silkworm was an exceptional phenomenon, and the possible genetic basis underlying it. Wilder range test of 31 strains of silkworm Bombyx mori for intrahemocoelical AcNPV infection led to the identification of 14 permissive strains and 17 nonpermissive strains, indicating that the intrahemocoelical infection of AcNPV to the silkworm was not a rare and isolated phenomenon. Productive infection was shown in permissive silkworms, by EGFP fluorescence in various tissues when expression of reporter gene controlled by a very late viral promoter polh. The viral titer in larval hemolymph of permissive silkworms increased and maintained at a higher level hundredfold more than the initial amount of virus, indicating viral replication. A series of genetic cross experiments suggested the existence of only one dominant host anti-AcNPV gene or a set of genetically linked genes, which prevent AcNPV infection in nonpermissive silkworm strain Qingsong and are absent in permissive silkworm strain Haoyue.