Abstract
Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
Highlights
Visceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world
The strain of E. coli expressing 503 antigen of Leishmania i. chagasi was kindly provided by Dr Mary Wilson (University of Iowa, USA) [1]
The batch adsorption experiment with Streamline Chelating showed that the optimal binding condition of 503 antigen was pH 8.0 in the presence of 1.625M NaCl
Summary
A disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. Methods The strain of E. coli expressing 503 antigen of Leishmania i. Chagasi was kindly provided by Dr Mary Wilson (University of Iowa, USA) [1].
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