Production, optimization, partial-purification and pyrolysis kinetic studies of exopolysaccharide from a native brown-rot fungi Fomitopsis meliae AGDP-2

Abstract

The fungal exopolysaccharides are one of the powerful and potent resources for medical applications. In the present study, thirteen white-rot fungi were screened for the production of exopolysaccharides (EPS) using five different cultivation media under submerged conditions and extracted using isopropanol. Maximum EPS produced was obtained from the new isolate AGDP-2. Upon morphological and molecular identification, the isolate was identified as Fomitopsis meliae AGDP-2 (GenBank Accession No.: MW888429.1). The production of EPS enhanced progressively and reached up to 10.4 g/L from 4.4 g/L after the optimization by One-factor-at-a-time (OFAT) method as well as upon statistical optimization using Plackett-Burman design (PBD) and response surface methodology - central composite design (RSM-CCD). Pyrolysis kinetic studies for EPS have been performed using thermogravimetric analysis (TGA) and its kinetic parameters such as activation energy were found to be ranging from 19.425 to 67.839 kJ/mol and pre-exponential factor ranged between 330.86 and 470,939.53 s−1.