Abstract
For the production ofcis,cis-muconic acidvia biocatalytic conversion reactions from a toxic cosubstrate, benzoic acid, a fed-batch process using computer-controlled DO-stat feeding was developed. The mutant strain ofPseudomonas putida BM014 producedcis,cis-muconic acid from benzoic acid with high conversion yield. More than 32 g/L ofcis,cis-muconic acid was accumulated in 42h and a productivity of 1.4 g/(L·h) was achieved.
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