Abstract
Objectives: This study aimed to obtain an optimum method for zincyeast production. Materials and Methods: Production of zinc-yeast was prepared using Yeast Extract Peptone Dextrose (YEPD) as a culture medium. Yeast that had been inoculated into culture media was incubated with a shaker at room temperature for 84 hrs, then zinc sulfate was added with various concentrations of 200, 300, and 400 mg/L, and then incubation continued for 24 hrs. The obtain yeast biomass was dried with a Freeze-dryer and analyzed for total accumulated zinc and protein content. Determination of zinc was using atomic absorption spectrophotometry (AAS) at a wavelength of 196 nm, whereas protein content was determined with Bradford method at a wavelength of 595 nm using UV-Vis spectrophotometer. Results: Zinc-yeast with the addition of 200 mg/L zinc sulfate obtained 0.3111 g that contains 2228.60 μg/g of zinc and 0.8515 mg/L of protein. While zinc-yeast with the addition of 300 mg/L zinc sulfate obtained 0.3704 g that contains 2458.30 μg/g of zinc and 0.8682 mg/L of protein. And the last, zinc-yeast with the addition of 400 mg/L zinc sulfate obtained 0.2370 g that contains 2648.43 μg/g of zinc and 0.8935 mg/L of protein. Conclusion: Production of zinc-yeast with the highest zinc and protein content can be obtained by adding 400 mg/L zinc sulfate solution to the stationary phase culture of S. cerevisiae. The modified zinc-yeast production method is simple and can be used for the production of functional food products.
Highlights
The world is being overwhelmed in managing the severe acute syndrome respiratory coronavirus 2 (SARS-CoV-2) as known as coronavirus disease 2019 (COVID-19)
Zinc can be obtained from various sources, including from yeast fermentation which is abundant with protein, amino acids, and peptides.[2]
Media was incubated using a shaker (150 rpm) at room temperature for 84 hrs. 12.5 mL of zinc sulfate with various concentrations of 200, 300, and 400 mg/L were added after 84 hrs of incubation, and incubation continued for 24 hrs with shaker (150 rpm)
Summary
The world is being overwhelmed in managing the severe acute syndrome respiratory coronavirus 2 (SARS-CoV-2) as known as coronavirus disease 2019 (COVID-19). According to Yasui et al (2020), most of the patients suffering from this disease showed zinc deficiency. Patients with zinc deficiency experience are more complicated, especially if they stay in the hospital sufficiently long, which can build mortality.[1] The aftereffect of the examination with rats showed that the immune response was significantly decreased (more than 50%) in zinc deficiency sufferers. Zinc has a role in immune response, including the development of immune cells and gene expression in immune cells. Zinc has an important role in cell multiplication and separation, signal transduction, energy-related supplement digestion, body development, and improvement. Zinc can be obtained from various sources, including from yeast fermentation which is abundant with protein, amino acids, and peptides.[2]
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