Abstract
Hemicellulose consists of non-cellulosic polysaccharides, with xylans and mannans as their main examples. In nature, xylan can be first degraded to xylooligosaccharides and finally to xylose by certain microorganisms. White-rot fungi basidiomycetes Pleurotus sp. BCCB068 and Pleurotus tailandia were used to degrade oat-spelts xylan under submerged fermentation for a period of 40 days. The study obtained activities of endo-1,4-β-xylanase and β-xylosidase and determination of xylan products by degradation. The fungi reached significant levels of xylan degradation by Pleurotus sp. BCCB068 (75.1%) and P. tailandia (73.4%), following formations of xylooligosaccharides and sugar monomers. These Pleurotus strains proved to be a feasible alternative for biotechnological processes related to degradation of hemicellulose sources
Highlights
Hemicellulose is one of the most abundant polysaccharides in nature present in agricultural biomass waste, and cannot be converted to simple monomeric sugars due to its recalcitrant nature
P. tailandia presented higher activity of xylanase on 20th day (0.25 U mL-1); very low values of this enzyme have been shown during this period (Figure 2)
Activities of xylanase, β-xylosidase and pH produced by P. tailandia during 40 days under submerged fermentation using xylan as carbon source
Summary
Hemicellulose is one of the most abundant polysaccharides in nature present in agricultural biomass waste, and cannot be converted to simple monomeric sugars due to its recalcitrant nature. The major component of hemicellulose in plant cell walls, is the second most abundant polysaccharide after cellulose (TAN et al, 2008). Xylan polymer consists of a main chain of β-1,4- linked D-xylose residues or some substitutes including L- arabinose, D-glucose, D-galactose, Dmannose, D-glucuronic acid, 4-O-methyl glucuronic acid D-galacturonic acid residues, as well as in less proportion the L-rhamnose, L-fucose and various O-methylated neutral sugars (SUN et al, 2000). The complete degradation of this complex structure depends on different enzymes acting in synergism endo-β-1,4-xylanases hydrolyze β-1,4-. Some other specific enzymes such as α-Larabnofuranosidase, α-glucuronidase, as well as several esterases have a cooperative function into the complete degradation of xylan (TENKANEN et al, 1996)
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