Abstract
This study aimed to optimize the production of xylanase, under submerged conditions using Fusarium oxysporum. Firstly, it was carried out the selection of the best source of carbon from agricultural waste, such as soybean husk, cassava peel, pineapple crown, corn straw, rice husk, bacaba, barley bagasse and, corncob. They were milled and sieved with a maximum granulation of 1 mm. The cultures were carried out by 132 hours 30 °C and 180 rpm. Among the agroindustrial residues, corn straw was the one that stood out with a greater enzymatic activity of 12.6 U/mL. In the second step, it was used Plackett-Burman design to screen the nutrients sources important to xylanase production. Thus, independent variables significant were urea and MgSO4. These variables selected by Plackett-Burman were then used in a Central Composite Rotational Design present activity of 26.6 U/mL and the predicted was 34.5 U/mL. So, the xylanase production by F. oxysporum can be optimize using corn straw, a low-cost waste found in large quantities.
Highlights
IntroductionPlant cell walls are composed of polysaccharides, lignins, proteins that together form a complex structure composed of different arrangements and interconnections forming a polysaccharide block, these arrangements and the interconnections of structural polysaccharides inside the cell walls are very restricted to enzyme access, explaining why these glycan compounds are recalcitrant to biological degradation [1]
Plant cell walls are composed of polysaccharides, lignins, proteins that together form a complex structure composed of different arrangements and interconnections forming a polysaccharide block, these arrangements and the interconnections of structural polysaccharides inside the cell walls are very restricted to enzyme access, explaining why these glycan compounds are recalcitrant to biological degradation [1].Cellulose is the most challenging material to degrade and deconstruct into carbohydrate monomers
This study aimed to evaluate the production of xylanase by F. oxysporum in different residual agro-industrial biomass and estimate the number of enzymes produced from the best chemical composition of the culture medium under submerged conditions
Summary
Plant cell walls are composed of polysaccharides, lignins, proteins that together form a complex structure composed of different arrangements and interconnections forming a polysaccharide block, these arrangements and the interconnections of structural polysaccharides inside the cell walls are very restricted to enzyme access, explaining why these glycan compounds are recalcitrant to biological degradation [1]. Cellulose is the most challenging material to degrade and deconstruct into carbohydrate monomers. Hemicellulose is the second-largest component of the plant wall after cellulose. It comprises a group of heterogeneous polysaccharides, representing 20-35% of the total dry weight of the biomass, consisting essentially of a large fraction of xylan with a linear β-(1-4)-D-xylose skeleton [2, 3]. The enzymatic hydrolysis of these lignocellulosic materials by cellulolytic and hemicellulolytic enzymes is the most promising approach to obtain high yields of products vital for economic success. Hemicellulose mainly requires the enzymes endo-1,4-β-xylanase (EC 3.2.1.8) and 1,4-β-xylosidase (EC 3.2.1.37), where the first enzyme acts
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