Abstract
A method has been developed to obtain virus-free sweet pepper plants from apical meristems by in vitro grafting. The shoot tips (0.4–0.8 mm) excised from virus-infected plants in a green house were grafted onto decapitated rootstock seedlings. The shoot tip was an apical meristem with two to four leaf primordia. Shoot development was observed in 2–83% of grafted shoot tips. By this method, virus-free plants were produced from the mother plants infected with Tobacco mosaic virus (TMV) or with Tomato spotted wilt virus (TSWV) or with both viruses.
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