Abstract
Salivary plasminogen activator from the vampire bat Desmodus rotundus (DSPA α1) is a promising new thrombolytic agent. Continuous growth of a stably transfected, methotrexate amplified, dhfr − CHO cell line yields up to 60 mg l −1 of DSPA α1. Utilizing an engineered baculovirus 10 mg l −1 were produced in batches of Sf 9 insect cells. Recombinant DSPA α1 is purified from both sources using a one-step purification protocol. Although differences in glycosylation were detected, enzymatic activity and fibrin cofactor dependency are unaffected when DSPA α1 derived from the two expression systems is compared.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
