Abstract
A method to produce transgenic Camelina sativa plants in cvs. PI650159 and PI650161 was developed. Micropropagated shoot meristem cultures were established from in vitro germinated seedlings and used as target tissues for Agrobacterium-mediated transformation. A plasmid harboring enhanced green fluorescent protein, β glucuronidase and neomycin phosphotransferase II genes were used to optimize parameters for transgenic plant production. Kanamycin at 40 mg·l-1 was effective in suppression of non-transformed cells while permitting growth of transgenic tissues. Shoot apical meristems co-cultivated with Agrobacterium exhibited stable enhanced green fluorescence protein (EGFP) and β glucuronidase (GUS) expression after culture on plant regeneration medium. We observed transformation efficiencies of 53.33% in cv. PI650159 and 98.33% in cv. PI650161. The presence of transgenes in both cultivars was confirmed by PCR, while quantitative real-time PCR detected single copy integration in Pl650161 and two copy integration in Pl650159. Transgenic plants exhibited EGFP and GUS expression in all tissues including shoots, leaves, buds, floral organs, seeds, and pods. Our results demonstrate a simple and efficient technique using apical shoot meristems for production of transgenic C. sativa plants that can be used for transfer of desirable traits.
Highlights
IntroductionWith oil content in seeds ranging from 38% - 43%, and the vast majority of fatty acids (>90%) being polyunsaturated [1] [2], biodiesel derived from C. sativa is well described
Our results demonstrate a simple and efficient technique using apical shoot meristems for production of transgenic C. sativa plants that can be used for transfer of desirable traits
We report for the first time, the use of apical meristematic tissue as targets for Agrobacterium-mediated transformation in C. sativa
Summary
With oil content in seeds ranging from 38% - 43%, and the vast majority of fatty acids (>90%) being polyunsaturated [1] [2], biodiesel derived from C. sativa is well described. Both seed oil and biodiesel produced from the species are extensively tested and the fuel used in engine trials with promising results [3] [4]. Since the plant is established as a potential biofuel feedstock, it has been extensively tested for fatty acid composition and oil profiles, as well as crop improvement efforts to enhance agronomic qualities such as drought resistance met with success [5] [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
