Abstract
A new method for the production of tocopherols by safflower ( Carthamus tinctorius) cell culture has been developed. The main tocopherol produced is α-tocopherol which has the strongest vitamin E activity among tocopherol analogues. In a time course experiment tocopherol production showed a secondary metabolic pattern rather than a primary one. Better cell lines in terms of both growth rate and amounts of tocopherols produced were obtained by selection using various growth regulators and media additives. In addition, tocopherol production was effectively stimulated by administration of biosynthetic precursors. In particular, phytol increased the total tocopherol content by some 18-fold, i.e. 63.6 mg per 100 g dry weight, and the α-tocopherol content by some 11- fold i.e. 28.8 mg per 100 g dry weight.
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