Production of Thermophilic Endo-β-1,4-xylanases by Aspergillus fumigatus FBSPE-05 Using Agro-industrial By-products

Abstract

In the present paper, endo-β-1,4-xylanase production by Aspergillus fumigatus was evaluated in solid-state fermentation using low-cost substrates such as sugarcane bagasse (SCB), brewer's spent grain (BSG), and wheat bran (WB). The partial characterization of the crude enzyme was also performed. In the experimental conditions, the highest levels of endo-β-1,4-xylanase production by A. fumigatus FBSPE-05 occurred within 8days incubation when using SCB/liquid medium at 1:2 ratio (219.5U g(-1)) and 4days incubation when using WB/liquid medium at 1:1 ratio (215.6U g(-1)). Crude enzyme from this last condition was used to enzyme characterization, showing best enzyme activity at 60°C and pH6.0, which suggests a thermophilic endoxylanase. The crude enzyme retained 73% of its activity after 1h at 60°C, and zymogram has shown three bands of endo-β-1,4-xylanase activity, with different molecular masses. A. fumigatus FBSPE-05 was able to grow and produce good levels of endo-β-1,4-xylanase using agro-industrial by-products, making this strain worthy for further investigation. To our knowledge, this is the first study reporting the use of SCB and/or BSG as sole substrates for endoxylanase production by solid-state fermentation using A. fumigatus.