Production of the anti-inflammatory compound 6-O-palmitoyl-3-O-β-D-glucopyranosylcampesterol by Callus cultures of Lopezia racemosa Cav. (Onagraceae).

Roberta Salinas,Jesús Arellano-García,María Luisa Garduño-Ramírez,Patricia Castillo-España,Silvia Marquina,Irene Perea-Arango,Laura Alvarez,Alejandro Zamilpa

doi:10.3390/molecules19068679

Roberta Salinas, Jesús Arellano-García + Show 6 more

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https://doi.org/10.3390/molecules19068679

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Abstract

Lopezia racemosa Cav. is a plant used in Mexican traditional medicine to heal inflammatory diseases. From this plant we isolated the novel compound 6-O-palmitoyl-3-O-β-D-glucopyranosylcampesterol (1) and 6-O-palmitoyl-3-O-β-D-glucopyranosyl-β-sitosterol (2), previously reported to have cytotoxic activity on several cancer cell lines. We evaluated the anti-inflammatory activity of 1 in vivo by mouse ear edema induced with 12-O-tetradecanoylphorbol-13-acetate (TPA) and 57.14% inhibition was observed. The aim of our study was to obtain callus cultures derived from this plant species with the ability to produce the compounds of interest. Callus cultures were initiated on MS basal medium amended with variable amounts of naphthaleneacetic acid (NAA), or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 6-benzylaminopurine (BAP). Ten treatments with these growth regulators were carried out, using in vitro germinated seedlings as source of three different explants: hypocotyl, stem node, and leaf. Highest yield of 1 was observed on callus derived from leaf explants growing in medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BAP. Selected callus lines produced less 1 than wild plants but the in vitro cultured seedlings showed higher production. So we conclude that it could be attractive to further investigate their metabolic potential.

Highlights

Plants are an important source of new products with medicinal value for drug development.Nowadays several chemicals derived from plants are drugs currently used in many countries around the world [1]
Our results showed that the extract with higher anti-inflammatory activity was SMD, with 58.3% of inhibition (Table 1)
Later on this fraction was subjected to rechromatography and our novel compound 1 was obtained mixed with 6-O-palmitoyl-3-O-β-D-glucopyranosyl-β-sitosterol (2)

Summary

Introduction

Plants are an important source of new products with medicinal value for drug development. Nowadays several chemicals derived from plants are drugs currently used in many countries around the world [1]. Some strategies using in vitro systems had been extensively studied to improve the production of plant chemicals with anticancer activity such as taxol, docetaxel, camptothecin, podophyllotoxin, vincristin and vinblastine. These substances have complex chemical structures and despite advances in synthetic chemistry its production still depends directly or indirectly on semi-synthesis from biological sources [4]

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