Abstract
The aim of this work was to test a protocol for producing tetraploid plants of Gentiana. Tetraploid plants were produced by growing in vitro cultures of Gentiana triflora var. japonica ‘Royal Blue’ in the presence of oryzalin for 4 weeks. A population of plants was regenerated from these in vitro cultures and transferred to the greenhouse. The treated plants were screened for higher ploidy level on the basis of leaf thickness and further confirmation of polyploidy was provided by measuring guard cell lengths. The tetraploid nature of one plant with larger than normal guard cells was confirmed by flow cytometry and chromosome counts. The mean nuclear DNA contents of 2C and 4C nuclei were 9.26 pg and 17.77 pg DNA, respectively. Mitotic chromosome counts from diploid and tetraploid plants were 2n=26 and 2n=52 respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: New Zealand Journal of Crop and Horticultural Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
