Abstract
Due to the depletion of fossil fuel resources and concern about increasing atmospheric CO2 levels, the production of microbial oil as source for energy and chemicals is considered as a sustainable alternative. A promising candidate strain for the production of microbial oil is the oleaginous yeast Schwanniomyces occidentalis CBS 2864. To compete with fossil resources, cultivation and processing of S. occidentalis requires improvement. Currently, different cell wall disruption techniques based on mechanical, chemical, physiological, and biological methods are being investigated using a variety of oil producing yeasts and microalgae. Most of these techniques are not suitable for upscaling because they are technically or energetically unfavorable. Therefore, new techniques have to be developed to overcome this challenge. Here, we demonstrate an effective mild enzymatic approach for cell disruption to facilitate lipid extraction from the oleaginous yeast S. occidentalis. Most oil was released by applying 187 mg L−1 tailor-made enzymes from Trichoderma harzianum CBS 146429 against the yeast cell wall of S. occidentalis at pH 5.0 and 40 °C with 4 h of incubation time after applying 1 M NaOH as a pretreatment step.
Highlights
Due to the expanding biobased economy, the interest in microbial oil is increasing (Athenaki et al 2018; Sitepu et al 2014a, b)
In this study we present a production method for tailor-made enzymes (TMEs) that are capable of degrading the cell wall of the oleaginous yeast S. occidentalis after a non-thermal pretreatment step
Bioreactor cultivation A cultivation procedure using bioreactors was performed to produce tailor-made enzymes (TMEs) by T. harzianum fed with wet biomass of non-fat S. occidentalis grown on yeast-extract peptone dextrose (YPD)
Summary
Due to the expanding biobased economy, the interest in microbial oil is increasing (Athenaki et al 2018; Sitepu et al 2014a, b). The possibility of economically feasible bio-oil production is being investigated in algae, yeasts, and filamentous fungi (Remmers et al 2017; Hao et al 2016; Sitepu et al 2014b; Ageitos et al 2011). An economically viable production process will require improvements to oil yield through optimization of cultivation and processing, as well as isolation of multiple coproducts in a biorefinery setup, indicating the need for mild downstream processing techniques. Efficient extraction of these oils is one of the bottlenecks in ensuring that the use of microbial oil is economically viable
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