PRODUCTION OF T7 RNA POLYMERASE ENZYME WITH RECOMBINANT DNA TECHNOLOGY

Abstract

T7 RNA polymerase is an enzyme that performs RNA synthesis using the DNA template. RNA polymerases carry out the process of RNA synthesis using the template of DNA, while T7 RNA polymerase is an enzyme found in the genome of the T7 bacteriophage [1]. T7 RNA polymerase is an enzyme used especially in in vitro (extracellular) transcription experiments. This enzyme initiates RNA synthesis from the DNA template and creates the RNA molecule. T7 RNA polymerase is known for its high specificity and efficiency. It can synthesize RNA more quickly and effectively compared to other RNA polymerase enzymes. An important feature of the T7 RNA polymerase is its low requirement for additional protein factors. While some RNA polymerase enzymes require the presence of various protein factors for RNA synthesis, T7 RNA polymerase is less dependent on these factors. This feature enables T7 RNA polymerase to be used more simply and quickly [2,3].