Abstract
Several yeasts, Geotrichum candidum, Yarrowia lipolytica, Kluyveromyces lactis, Debaryomyces hansenii, and Saccharomyces cerevisiae, were studied for their ability to generate volatile sulfur compounds. These yeasts were cultivated on a synthetic culture medium supplemented with a biosynthetic precursor. With S-methylmethionine, dimethylsulfide (DMS) was the major sulfur compound produced. l-methionine promoted the synthesis of a wider spectrum of volatile sulfur compounds, methanethiol (MTL), DMS, dimethyldisulfide (DMDS), dimethyltrisulfide (DMTS) and S-methylthioacetate (MTA). Enzymatic activities possibly involved in sulfur compound synthesis were also investigated. Important l-methionine-transaminase activities, and also l-methionine-demethiolase activities were detected in cellular extracts. Their possible role in the generation of sulfur compounds and related biosynthetic pathways are also discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have