Abstract
The production of sophorose lipids increased with increasing concentrations of both safflower oil and glucose, and was profoundly influenced by the concentration of yeast extract. A high concentration of sophorose lipids (about 135 g/L) was obtained (in a 1‐L Bellco stirred reactor) when the medium consisted of 10% glucose, 10.5% safflower oil, 0.1% urea, and 0.25–0.3% yeast extract. A similar yield of sophorose lipids also was obtained in a 20‐L bioreactor. About 50% of the apolar sophorose lipid 1′,4″‐lactone 6′,6″‐diacetate (SL‐1) was found in the mixture of sophorose lipids produced under these conditions.
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