Abstract
Summary As a step towards the development of a somatic hybridization system, protoplast fusion between Hordeum vulgare L. and Hordeum bulbosum L. was performed. In H. vulgare an embryogenic cell suspension was chosen as a protoplast donor. In H. bulbosum the npt II gene was introduced into protoplasts and then one of the resulting lines was used for hybridization experiments. Protoplasts were fused by a newly developed method using polyethyleneglycol (PEG) and Millicell inserts. Selection of hybrid cells was based on iodoacetoamide (IOA) inactivation and antibiotic resistance. RFLP analysis using rDNA probes confirmed the production of somatic hybrid cell lines between H. vulgare and H. bulbosum .
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
