Abstract
SUMMARY: Ultrasonic treatment of double-walled cysts of Schizopyrenus russelli led to the removal of the outer wall. These single-walled cysts excysted readily in aqueous extract of Escherichia coli, as did normal double-walled cysts. There was a marked delay in the excystment of single-walled cysts obtained by ultrasonic treatment of the normal cysts treated with p-chloromercuric benzoate (p-CMB) or emetine, similar to that obtained with normal cysts. The action of p-CMB was annulled by reduced glutathione. These findings suggest that the sites of action of excystment-inducing and excystment-blocking agents are on or inside the inner cyst wall and that these agents are able to permeate the outer cyst wall.
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