Abstract
Abstract Oleaginous yeasts seem to be the promising source for the production of lipid. In this context Rhodotorula glutinis was investigated utilizing an agro-industrial waste cassava peel. Medium optimization was performed by response surface methodology (RSM) with Box-Behnken design under submerged fermentation to improve the lipid yield. The results of RSM revealed that the highest lipid production of 58% lipid/dry biomass may be achieved when the culture was grown for 3 days at 30 °C under 120 rpm shaking, pH 5.45 in a medium containing 12% (w/v) cassava peel and 8.03% (v/v) yeast inoculum. Scanning electron microscopic studies confirmed complete colonization of starch granules by yeast. The process was further up scaled in a 7 L bio-reactor and an yield increment of 9% was observed. Overall, the above results clearly displayed the potential of R. glutinis to utilize cassava peel waste for the single cell oil (SCO) production.
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