Abstract
Production of Rifamycin with Amycolatopsis mediterranei (MTCC14) was studied using carbon and locally available cheaper nitrogen sources. A. Mediterranei gave initial yield of 650 mg/l with standard fermentation medium. This was improved to 1400 mg/l by using various Carbon, Nitrogen sources and optimizing various cultural conditions. Glucose, Ammonium sulphate and combination of soya bean and pea nut meals were found to induce more Rifamycin production in 7 days with a pH of 7.2, temperature 28 °C.
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