Abstract

To date, a number of processes have been reported to be in commercial scale operation for producing plant cell products such as red dye, shikonin, and alkaloid berberine. However, the cultivation of plant cells and tissues in a large-scale bioreactor is still not as well developed as microbial fermentation. Some technical difficulties encountered, such as slow metabolic rate and heterogeneity in cell population, are due to the biological characteristics of plant cells. Other problems, such as foaming and wall adhesion [1], viscosity of cell culture [2] and shear sensitivity of the cells [3], are related to engineering or bioreactor design [4]. Here, we address the effects of aeration and light irradiation on anthocyanin production by suspension culture of Perilla frutescens in bioreactors.

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