Production of Reactive Oxygen Species via Nanobubble Water Improves Radish Seed Water Absorption and the Expression of Aquaporin Genes.

Abstract

Nanobubbles (NBs) stimulate seed germination; however, the mechanism of the promotion effect of NBs remains unclear. The impact of NBs on seed water absorption was investigated; we subsequently studied the genes associated with the response of radish seeds to NB water and used RNA sequencing to generate their expression profiles, especially those of aquaporin genes. NB water significantly promoted germination. The times at which 50% of the germinating seeds achieved germination (T50) for the submerged radish seeds in NB and control water were 11.6 and 17.4 h, respectively. NB water-germinated radish seeds showed a water uptake rate coefficient that was 15% higher than that of those germinated in control water. Through GO enrichment and cluster analyses, it was evident that NB water significantly increased the level of expression of the genes associated with the following activities: oxidoreductase, peroxidase, and antioxidant. Our results demonstrated that NB water increases the water uptake rate of radish seeds via two mechanisms. The NB water-produced exogenous hydroxyl radical (•OH) increases the seed coat's water permeability and enhances cell wall loosening, and NB water increases the aquaporin gene expression level of radish seeds.