Abstract
(R)-hydroxyalkanoic acids (R-HAs) are valuable building blocks for the synthesis of fine chemicals and biopolymers because of the chiral center and the two active functional groups. Hydroxyalkanoic acids fermentation can revolutionize the polyhydroxyalkanoic acids (PHA) production by increasing efficiency and enhancing product utility. Modifying the fermentation conditions that promotes the in vivo depolymerization and secretion to fermentation broth in wild type bacteria is a novel and promising approach to produce R-HAs. Wood extract hydrolysate (WEH) was found to be a suitable substrate for R-3-hydroxybutyric acid (R-3-HB) production by Burkholderia cepacia. Using Paulownia elongate WEH as a feedstock, the R-3-HB concentration in fermentation broth reached as high as 14.2 g/L after 3 days of batch fermentation and the highest concentration of 16.8 g/L was obtained at day 9. Further investigation indicated that the composition of culture medium contributed to the enhanced R-3-HB production.
Highlights
(R)-hydroxyalkanoic acids (R-HAs) are valuable building blocks for the synthesis of fine chemicals and biopolymers because of the chiral center and the two active functional groups
Industrial production of polyhydroxyalkanoic acids (PHA) has been based on the intracellular synthesis, which is different from polylactic acid (PLA) production (Buyondo and Liu 2011)
In vivo depolymerization of PHA requires the biomass with PHA accumulated to be harvested from fermentation broth first, and PHA degradation is enhanced by modifying the pH or temperature (Lee et al 1999; Ren et al 2005)
Summary
(R)-hydroxyalkanoic acids (R-HAs) are valuable building blocks for the synthesis of fine chemicals and biopolymers because of the chiral center and the two active functional groups. Hydroxyalkanoic acids fermentation can revolutionize the polyhydroxyalkanoic acids (PHA) production by increasing efficiency and enhancing product utility. In vivo depolymerization of PHA requires the biomass with PHA accumulated to be harvested from fermentation broth first, and PHA degradation is enhanced by modifying the pH or temperature (Lee et al 1999; Ren et al 2005). Production of R-HAs by wild-type bacteria fermentation through modifying the culture conditions is a very promising and innovative approach (Kawata et al 2012; Ren et al 2010). KM-1 in 200 mL Erlenmeyer flasks culture (Kawata et al 2012)
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