Abstract
ACCORDING to Doerr1, Sh. dysenteriœ fails to produce toxin in a synthetic medium, but regains its toxicity if transferred from the synthetic medium into alkaline broth. Braun and Cahn-Bronner2 showed that certain dysentery strains can utilize ammonium as source of nitrogen, and that they produce a weak toxin on media containing lactate as source of carbon, and ammonium as source of nitrogen. From a medium with this composition Wichmann3 produced a toxin with a minimal lethal dose of 1.0–2.0 c.c. on rabbits of about 1,000 gm. weight.
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