Production of polyhydroxyalkanoates (PHA) by pseudomonas from waste products

Abstract

Polyhydroxyalkanoate commercial manufacture is hampered by high production costs (PHAs). Up to 50% of the total process expenses might be attributed to the medium used for cultivation. With this research, the researchers hoped to determine if acidogenic fermentation of food waste may yield volatile fatty acids as low-cost carbon sources for bacterial growth essential to make polyhydroxyalkanoates (PHAs). The bacterium Pseudomonas pseudoflava was used to examine the generation of polyhydroxyalkanoates (PHA) from waste food at varied carbon concentrations (4-55g/l). Bacteria produced the most PHA at a concentration of 19 g/l (52.5 percent) and removed the most carbon at a concentration of 7 g/l (67.6 percent). Various analytical methods were used to examine the synthesized PHA's structure, molecular weight, and thermal characteristics, among other things. According to the results of this study, organic carbon persisting in wastewaters may be degraded and converted into PHA by using P. pseudoflava.