Abstract
Polyhydroxyalkanoates (PHAs) are polyesters naturally synthesized by numerous bacteria as intracellular energy storage materials during unbalanced growth. These polyesters find many applications in medicine, veterinary practice, tissue engineering, food packaging, etc. In the present study, spent wash and oil mill soil samples were screened for the isolation of bacteria producing polyhydroxyalkanoates. Different bacterial isolates were screened by PCR using primers designed for Bacillus megaterium. Isolate-4 reacted positively giving amplicons similar in size to that of Bacillus megaterium. Based on 16S rRNA gene sequences, isolate-4 was identified as Paenibacillus durus. Nitrogen limited mineral salt medium with fructose as chief carbon source was used for production of Polyhydroxybutyrate (PHB) in shake flask studies. The effect of various carbon and nitrogen sources on PHB production was evaluated, with respect to yield and PHB content. The study revealed that fructose and peptone gave better PHB accumulation and growth rates for the isolate and the reference strain, and hence, were the best among the carbon and nitrogen sources used. Further studies are needed to optimize the production conditions.
Highlights
Polyhydroxyalkanoate (PHA) is one of the microbial polymers which accumulate in bacteria as intracellular storage granules, in the presence of excess carbon sources and limited nitrogen source [1]
3-hydroxybutyrate (PHB), a class of PHA is insoluble in water, resistant to ultraviolet radiation, and is impermeable to oxygen, and finds many industrial applications, including use as food packaging material
Accumulation of Poly 3-hydroxybutyrate (PHB) as hydrophobic inclusions is observed in the cytoplasm of Bacillus megaterium, and in many other Gram positive and Gram negative bacteria [6,7,8]
Summary
Polyhydroxyalkanoate (PHA) is one of the microbial polymers which accumulate in bacteria as intracellular storage granules, in the presence of excess carbon sources and limited nitrogen source [1]. PHB accumulating bacteria from oil mill soil were isolated, identified, and characterized. An attempt was made to produce effective amount of PHB using different carbon and nitrogen sources in mineral salt medium. PHB production in shake flasks was studied using the basal mineral salt medium, with fructose as carbon source [10].
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