Production of polyclonal antibodies against the BYMV isolate infecting gladiolus crop naturally and development of polyclonal antibodies based serological diagnostic system

Abstract

In this study a bean yellow mosaic virus isolate infecting gladiolus crop with leaf mosaic and color breaking symptoms on flower was characterized based on mechanical transmission, symptomatology on hosts, and viron particle structure and capsid protein molecular weight determination. The virus isolate was successfully purified from the propagating host and purified viron particles were used for the production of polyclonal antibodies for the development of a cost effective and sensitive serological diagnostic system. The produced polyclonal antibodies would be useful for the assessment of BYMV infection in gladiolus crop and seed materials.