Abstract
Formulation of effective and environmental friendly bioherbicides depends on the type of fermentation medium used for the production of phytotoxic metabolites. The effect of biomass, colony forming unit and the phytotoxic metabolite produced from the biphasic fermentation was carried out, while the phytotoxic metabolite was tested in vivo and in-vitro on Echinochola crus-galli and dicotyledonous Chromolaena odorata. The mutant strain of Lasiodiplodia pseudotheobromae C1136 (Lp90) produced the highest amount of conidia and the largest necrotic area on the two tested weeds when compared to its wild strain in the different biphasic media combinations. The study revealed that the biphasic system containing PDB + rice produced the highest bioherbicidal activities. Therefore, the phytotoxic metabolites from strain C1136 are suggested for large scale production of bioherbicides for the management of weeds in conventional farming to improve yield and enhance food security.
Highlights
The use of microorganisms as biological control agents has been considered an important tool in integrated pest management (IPM) because the use of synthetic herbicides has posed a serious threat to food safety, the ecosystem, environmental and health hazards, there is a need for safe and effective bio-herbicides (Barranco Florido et al, 2002; Adetunji and Oloke, 2013; Adetunji et al, 2017a, b).Kayode and Ige (1998) discovered that C. odorata (L) and T. procumbens L. are one of the major weeds that constitute a major impediment to agricultural and natural ecosystem in Nigeria
The result obtained from the biomass produced during the biphasic fermentation showed that media containing potatoes dextrose broth (PDB) plus rice (PDB + rice) had the highest while the lowest was obtained in yeast malt broth plus millet (YMB +Millet) media
The effect of the phytotoxic metabolite produced from the biphasic fermentation tested on weed from leaves of monocotyledon (Echinochola crus-galli) and dicotyledonous (Chromolaena odorata) showed that the phytotoxic metabolites from the mutant strain C1136 Lp 90 produced the largest necrotic area on the two tested weeds at all the teste concentrations
Summary
Kayode and Ige (1998) discovered that C. odorata (L) and T. procumbens L. are one of the major weeds that constitute a major impediment to agricultural and natural ecosystem in Nigeria. Echinochloa crus galli is one of the most devastating weeds commonly associated with rice. Biphasic fermentation system combines the benefits of high biomass production in liquid fermentation and production of stable and hydrophobic aerial conidia on a solid substrate (Veloorvalappil et al, 2015). The importance of biphasic fermentation, when compared to other fermentation systems, include: prevention of contaminants that might be present from the culture stock, promotion increased the competitiveness of the fungus, ensuring a uniform colonization of the solid substrate, production of conidia is faster, reducing the incubation time and economizing physical space (Vladimir et al, 2014)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
