Abstract

AbstractThis paper discusses the effect of the utilization of Bacillus megaterium in the microbial solubilization process where poultry bones or ash were used as a source of renewable phosphorus. The process was performed in a large scale laboratory. The pH of the solution decreased during one-week solubilization, which had a direct influence on the increased concentration of phosphorus determined in the solution. It was proved that the phosphorus concentration in the solution was significantly correlated with the biomass concentration and pH. The trial allowed verification of the suitability of the method to prepare two P fertilizers: one based on poultry bones and one on ashes. The elemental analysis of their composition suggests that the bones are a P-bearing resource with properties better than ash. That enables for more efficient scaling-up the solubilization although the concentration of total phosphorus was comparable in both cases. The total amount (100%) of phosphorus was present in a form available to plants in the formulation based on the poultry bones, while 64% of plant available phosphorus was present in the formulation based on the ash. The concentration of Cd was significantly lower in the case of fertilizer based on bones with respect to ash.

Highlights

  • This paper discusses the effect of the utilization of Bacillus megaterium in the microbial solubilization process where poultry bones or ash were used as a source of renewable phosphorus

  • The specific growth rate, μ, 1/day of B. megaterium was calculated using Equation 2 and 3: Production of phosphorus biofertilizer based on the renewable materials in large laboratory scale 895

  • In order to investigate the efficiency of the solubilization process and the bioavailability of phosphorus, two fractions of phosphorus present in the solids that remained after the solubilization process: ammonium citrate and water extracts, were determined according to Regulation (EC) No 2003/2003 of the European Parliament and of the Council relating to fertilizers

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Summary

Introduction

Abstract: This paper discusses the effect of the utilization of Bacillus megaterium in the microbial solubilization process where poultry bones or ash were used as a source of renewable phosphorus. The trial allowed verification of the suitability of the method to prepare two P fertilizers: one based on poultry bones and one on ashes. That enables for more efficient scaling-up the solubilization the concentration of total phosphorus was comparable in both cases. The total amount (100%) of phosphorus was present in a form available to plants in the formulation based on the poultry bones, while 64% of plant available phosphorus was present in the formulation based on the ash. The concentration of Cd was significantly lower in the case of fertilizer based on bones with respect to ash

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