Abstract
Egg yolk lipids were extracted and fractionated into neutral lipids and phospholipids using acetone followed by ethanol as eluting solvents to a column consisting of spray-dried egg yolk. This technique, named in situ solid phase extraction, can be performed with spray-dried egg yolk due to the properties of this material. The effect of temperature, flowrate and scale has been studied. Results suggest that the methodology is suitable for industrial implementation.
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