Production of Pharmaceutical Proteins by Transgenic Avian

Abstract

Transgenic avian has been expected to be an excellent transgenic bioreactor for the production of recombinant pharmaceutical proteins. However, successful transgenic bioreactors have been reported only in mammals. We have attempted to establish the generation method of transgenic avian using retroviral vectors for gene delivery. Laid quail embryos at the blastodermal stage were infected with a pantropic retroviral vector, and the embryos were hatched in vitro to generate G0 quails. The viral vector sequence was detected in the tissues of all G0 quails. The germ-line transmission efficiency of G0 quails mated with non-transgenic quails was more than 80%. Plural copies of the transgene were inserted into the genome of G1 transgenic progenies. The transgene expression was also detected in G1 and G2 transgenic quails.