Abstract
A breeding strategy for widening the germplasm of Brassica napus was proposed by introgression of the A r subgenome of B. rapa (A rA r) and C c of B. carinata (B cB cC cC c) into natural B. napus (A nA nC nC n). The progenies with 38 chromosomes that were derived from the self-pollinated seeds of pentaploid hybrids (A rA nB cC cC n) were used for further research. Some of the partial new-typed B. napus showed normal meiotic behavior, high portion of germinated pollen and normal embryological development. This indicates that the selected new-typed B. napus had a balanced genetic base. Molecular analysis showed that about 50% of the genome in the new-typed B. napus was replaced by A r and C c subgenome from B. rapa and B. carinata. Considering the genetic diversity among different lines of new-typed B. napus it was deduced that the introgression of the genomic components from B. rapa and B. carinata could widen the genetic diversity of rapeseed.
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