Production of Organic Acids by Yarrowia lipolytica

Abstract

AbstractWild-type strains of Yarrowia lipolytica secrete several organic acids like citric acid, isocitric acid, α-ketoglutaric acid, fumaric acid, malic acid or pyruvic acid, under certain circumstances from different carbon sources. An excess of carbon source together with limitation of growth causes the overproduction of these acids in most cases. Very high amounts of secreted acids (up to 200 g L−1) can be reached in some cases like citric acid and α-ketoglutaric acid, but other acids like fumaric acid and succinic acid are produced in low amounts (below 10 g L−1) by wild-type strains of this yeast. Several mutants were selected and recombinant strains have been constructed to improve the amounts of accumulated acid, the productivity or to change the spectrum of secreted acids. This review summarises the present state of knowledge on this field of overproduction of organic acids by the yeast Y. lipolytica.KeywordsSuccinic AcidPyruvic AcidYarrowia LipolyticaCitric Acid ProductionIsocitrate LyaseThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.